Proteintech, 85798-1-RR, Strep Tag II Recombinant monoclonal antibody

Size: 100ul The Strep Tag II (85798-1-RR) by Proteintech is a Recombinant antibody targeting Strep Tag II in WB, IF/ICC, IP, ELISA applications with reactivity to recombinant protein samples 85798-1-RR targets Strep Tag II in WB, IF/ICC, IP, ELISA applications and shows reactivity with recombinant protein samples. Tested Applications Positive WB detected in: Transfected HEK-293T cells Positive IP detected in: Transfected HEK-293F cells Positive IF/ICC detected in: Transfected HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Strep-Tag II consists of 8 amino acids with the sequence of Trp-Ser-His-Pro-Gln-Phe-Glu-Lys. Compared with Strep-Tag, Strep-Tag II has a higher binding affinity with streptavidin and can be constructed at the N-terminus or C-terminus of the target protein, providing greater flexibility. Specification Tested Reactivity: recombinant protein Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Strep Tag II Calculated Molecular Weight: 1 kDa Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Strep Tag II Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.