Sartorius, 24775-017, HA Ultrogel® - 500 mL

HA Ultrogel® is a composite material of cross-linked agarose and microcrystalline hydroxyapatite enclosed in the agarose matrix. The material shows Mixed Mode functionality based on cation exchange and metal affinity in the hydroxyapatite structure. A wide variety of applications include separation of proteins, peptides and nucleic acids, from laboratory to production scale.

HA Ultrogel® resin is a hydroxyapatite agarose composite resin for the separation of biomolecules from research and to manufacturing scale. Hydroxyapatite chromatography is considered to be a “pseudo-affinity” chromatography, or “Mixed Mode” ion exchange. It has proven to be an effective purification mechanism in a variety of processes, providing biomolecule selectivity complementary to more traditional ion exchange or hydrophobic interaction techniques.
Bulk resins are available in various quantities to support different scaling processes, ensuring flexibility and adaptability. Perfect for large-scale purification processes.
Features and Benefits
Unique selectivity and separation mechanism, complementary to traditional IEX and HIC
Protein purification in neutral, non-denaturating conditions
High porosity, easy cleaning
Applications
Purification of human serum proteins
Purification of basic proteins, of phosphate-dependent proteins and enzymes, and of DNA-dependent enzymes
Vaccine purification processes, e.g. Bordetella pertussis toxin
Separation of plant proteins such as lectins, glycoproteins, glycosidases, phospholipidases, sulfohydrolases, sphingomyelinases, transferases, trehalases and kinases

Specifications
Applications:
Application: Preparative protein purification

Compliance Information:
Quality Standards: CoA; RSF (upon request)

Dimensions:
Bead Size: 60-80 µm

General Specifications:
Flow Rate: < 100 cm/h
Resin Format: Bulk resin

Materials of Construction:
Base Matrix: Agarose

Operating Conditions:
Operating Pressure: < 2 barg (44psi)

Physicochemical Information:
Ligand: Hydroxyapatite
Reference Protein: Cytochrome C
Typical Dynamic Binding Capacity 10% per mL: > 7 mg/mL Cytochrome C

Product Information:
Brand: HA Ultrogel®
Pack Size: 1
Pack Volume: 500 mL
Resin Delivery Condition: 1M NaCl with 20% EtOH
Resin Type: Mixed Mode cation exchanger
What you receive: 1 bottle resin, instructions

Storage Conditions:
Storage Conditions: 20% EtOH/1M NaCl