Sartorius, PRC08X100MEPHCEL, MEP HyperCel PRC Column - 5 mL

MEP HyperCel provides a unique separation mechanism and selectivity for protein separations, with ligand-based immunoglobulin selectivity optimal for monoclonal antibody capture and intermediate purification. The MEP ligand facilitates the binding of immunoglobulins as well as other target proteins at neutral pH under moderate salt concentrations.

MEP HyperCel is a flexible Mixed Mode chromatography resin designed for capture and purification of antibodies and various recombinant proteins from laboratory to manufacturing scale. MEP HyperCel resin offers a no salt/low salt alternative to conventional HIC and can simplify processes and improve economics by removing the need for salt addition and following UF/DF steps. The MEP ligand is immunoglobulin-selective. Thus, MEP Hypercel can be effectively used for IgG capture and for enhanced DNA, HCP and aggregate removal. Elution is under mild conditions.
MEP HyperCel pre-packed columns are designed to simplify resin screening and selection, making them ideal for optimizing process steps. The RoboColumn® format is particularly suited for high throughput process development (HTPD), allowing for efficient and streamlined purification processes.
Features and Benefits
Unique separation mechanism and differentiated selectivity
Direct protein capture from a variety of feedstocks
No salt addition required for binding means a simpler process, lower costs and reduced waste
Stable toward harsh cleaning with NaOH
Applications
A no-salt/low-salt alternative to Hydrophobic Interaction Chromatography (HIC)
Monoclonal and polyclonal IgG capture and intermediate purification (aggregate, DNA and HCP removal)
Direct capture of recombinant proteins or enzymes from feedstock without need for pH or conductivity adjustments

Specifications
Applications:
Application: Resin screening and process optimization

Compliance Information:
Quality Standards: CoA; RSF

Dimensions:
Bead Size: 80 - 100 µm
Bed Height: 100 mm
Dimensions: 8 x 100 mm

General Specifications:
Flow Rate: 200-600cm/h
Resin Format: Pre-packed column

Materials of Construction:
Base Matrix: Rigid cellulose beads
Materials of Construction: Polypropylene

Operating Conditions:
Operating Pressure: < 3 barg (44psi)
pH Stability: 2 to 14 (short term), 2 to 12 (long term)

Physicochemical Information:
Bed Volume: 5 mL
Ligand: 4-mercaptoethylpyridine
Reference Protein: Human polyclonal IgG
Typical Dynamic Binding Capacity 10% per mL: ≥ 20 mg/mL hIgG at 6' residence time

Product Information:
Brand: MEP HyperCel
Pack Size: 1 column per pack
Pack Volume: 5 mL
Resin Delivery Condition: 1M NaCl with 20% EtOH
Resin Type: Mixed Mode anion exchanger
What you receive: 1 column, instructions

Storage Conditions:
Storage Conditions: 20% EtOH/1M NaCl