Sartorius, SR6HEA, HEA HyperCel - 600 µL

HEA HyperCel is a mixed-mode resin with a less hydrophobic hexylamine ligand allowing protein binding at a moderate salt concentration under neutral pH. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).

HEA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “Mixed Mode” mechanism, with a hexylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. HEA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).
HEA HyperCel pre-packed columns are designed to simplify resin screening and selection, making them ideal for optimizing process steps. The RoboColumn® format is particularly suited for high throughput process development (HTPD), allowing for efficient and streamlined purification processes.
Features and Benefits
High mechanical and chemical stability
Suitable for large-scale chromatography in a biopharmaceutical environment
Cross-linked cellulose base matrix
Applications
Protein purification at low ionic strength by direct hydrophobic capture
Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
Orthogonal separation mechanism to ion exchange or HIC

Specifications
Applications:
Application: HTPD resin screening

Compliance Information:
Quality Standards: CoA; RSF

Dimensions:
Bead Size: 80 - 100 µm
Bed Height: 30 mm
Dimensions: 5 x 30 mm

General Specifications:
Flow Rate: 16-1000 cm/hr
Resin Format: Pre-packed column

Materials of Construction:
Base Matrix: Rigid cellulose beads
Materials of Construction: Polypropylene

Operating Conditions:
Operating Pressure: < 3 barg (44psi)
pH Stability: 1 to 14 (short term), 2 to 12 (long term)

Physicochemical Information:
Bed Volume: 600 µL per column
Ligand: Hexylamine
Reference Protein: Bovine serum albumin (BSA)
Typical Dynamic Binding Capacity 10% per mL: 40-60 mg/mL BSA

Product Information:
Brand: HEA HyperCel
Pack Size: 1 row of 8
Pack Volume: 600 µL
Resin Delivery Condition: 1M NaCl with 20% EtOH
Resin Type: Mixed Mode anion exchanger
What you receive: 1 row of 8 columns

Storage Conditions:
Storage Conditions: 20% EtOH/1M NaCl