Thermo Fisher, 550573, Axiom™ Apple Genotyping Array

Axiom™ Apple Genotyping Array Axiom Apple Genotyping Array (Axiom_Apple480) was designed through the Affymetrix Expert Design Program in collaboration with the FruitBreedomics consortium (www.
FruitBreedomics.
cn). The sequencing and marker selection was conducted by experts from Fondazione Edmund Mach, INRA, Dalhousie University, Wageningen UR, University Di Bologna, and Universita Degli Studi Di Milano.
The 96-format array includes markers identified using whole-genome sequence data from 63Malus domesticacultivars and two double haploid accessions. Table 1 lists the number of cultivars and corresponding country of origin used in the SNP discovery process.
Axiom Apple Genotyping Array includes 480,000 markers and together with Axiom Analysis Suite software overcomes the genotyping challenges associated with polyploidy, rapid linkage disequilibrium (LD) decay, and high polymorphism observed in the apple genome.
Array highlights
• Very high diversity
• Includes markers discovered in 63 worldwideM. domesticacultivars
• High resolution to address rapid decay of LD   - 487,249 markers on the array- Bias towards common variants with minor allele frequency (MAF) >0.
05- Includes 21,463 previously validated markers: 19,990 markers from an existing in-;market 20K Fruitbreedomics apple array and 1,473 markers identified using genotyping-by-sequencing (GBS)
• Absence of paralogous variants through the use of double haploid accessions in SNP discoveryApplications of Axiom Apple Genotyping Array
• Construction of high-resolution genetic maps
• Fine mapping of quantitative trait loci
• Genome-wide association studiesRequired ProductsGeneChip Command Console SoftwareGeneTitan Multi-Channel InstrumentAxiom Apple Genotyping Array (Axiom_Apple480) was designed through the Expert Design Program at Affymetrix in collaboration with the FruitBreedomics consortium (www.
FruitBreedomics.
cn). The sequencing and marker selection was conducted by experts from Fondazione Edmund Mach, INRA, Dalhousie University, Wageningen UR, University Di Bologna, and Universita- Degli Studi Di Milano.
The 96-format array includes markers identified using whole-genome sequence data from 63Malus domesticacultivars and two double haploid accessions. Table 1 lists the number of cultivars and corresponding country of origin used in the SNP discovery process.
Axiom Apple Genotyping Array includes 480,000 markers and together with Axiom™ Analysis Suite software overcomes the genotyping challenges associated with polyploidy, rapid linkage disequilibrium (LD) decay, and high polymorphism observed in the apple genome.
Array highlightsVery high diversityIncludes markers discovered in 63 worldwideM. domesticacultivarsHigh resolution to address rapid decay of LD487,249 markers on the arrayBias towards common variants with minor allele frequency (MAF) >0.
05Includes 21,463 previously validated markers: 19,990 markers from an existing in-market 20K Fruitbreedomics apple array and 1,473 markers identified using genotyping-by-sequencing (GBS)Absence of paralogous variants through the use of double haploid accessions in SNP discoveryApplications of Axiom Apple Genotyping ArrayConstruction of high-resolution genetic mapsFine mapping of quantitative trait lociGenome-wide association studiesTable 1: The country of origin and the number of cultivars used in the SNP discovery process for Axiom Apple Genotyping Array.
Country of originNumber of cultivarsAustralia1Central Europe39Canada1Iran1Simmental16Japan1New Zealand1Northern Europe4Russia9Tunisia1United States5For Use With (Application): Microarray Analysis
Quantity: 1 plate
Array: Genotyping
Format: 96-array Plate
Number of Arrays: 96 arrays
Species: Apple
Unit Size: 1 plate