Thermo Fisher, 551041, Axiom™ Strawberry FanaSNP 50k Genotyping Array, 384-HT format

Axiom™ Strawberry FanaSNP 50k Genotyping Array, 384-HT format The Axiom FanaSNP Strawberry 50K Genotyping Array offers genome-wide coverage of polymorphic SNPs across the cultivated and wild octoploid strawberry species. It is a component of our Axiom Agrigenomics Genotyping solution where target genomic DNA is prepared and processed on the GeneTitan Multi-Channel instrument. The automated process include hybridization of the gDNA to this high-throughput microarray followed by staining, washing, imaging, and output of genotyping data for further analysis. The array is also available in mini-96 format (Cat. No. 551042).
The Axiom FanaSNP Strawberry 50K Genotyping Array was designed by the University of California, Davis Strawberry Breeding Program in collaboration with Affymetrix Expert Design Program1.
Array contentComprehensive content:850,000 subgenome-specific SNPs from diverse octoploid strawberry accessions from around the globe. Collectively, 446,644 of 850,000 marker probes produced QC-passing polymorphic SNP genotype clusters showing disomic (allopolyploid) segregation. The complete set of 446,644 validated probes is made available for public use.
Production-scale array design:the most performant set of 49,483 SNPs was tested and validated for production activities. 5,809 SNPs from the iStraw 35K array were maintained for connectivity to previous platforms.
Tied to multiple reference genomes:SNP positions ascertained by WGS alignment to theCamarosav1 reference genome2and updated to theUCD Royal Royce(FaRR1) reference genome3Globally represented and validated:variants discovered by WGS resequencing of 47 F. x ananassa, 24 F. chiloensis, and 22 F. virginiana individuals. Subgenome-specific marker performance variants were validated in a genetically diverse sample of 384 octoploid strawberry accessions.
ApplicationsMolecular genetics research and inferenceQTL discovery and genome-wide associationKinship and parentage analysisGenomic and molecular breedingGenomic prediction and selectionMarker-assisted selectionRelease of new cultivarsAccelerate and increase efficiency of cultivar developmentIdentify the presence of important markers in existing strawberry germplasmUse genetic information to identify germplasm that will perform well in specific growing regionsIntellectual property protection and verificationClonal source and purity confirmationValidation of parent-offspring relationshipsRequired productsGeneChip Command Console SoftwareGeneTitan Multi-Channel InstrumentAxiom Analysis Suite SoftwareAxiom Long Format Export Tool (AxLE)ReferencesHardigan et al. 2020;https://doi.
org/10.
3389/fpls.
2019.
01789Edger et al. 2019;https://doi.
org/10.
1038/s41588-019-0356-4Hardigan et al 2022;https://doi.
org/10.
1101/2021.
11.
03.
467115;https://phytozome-next.
jgi.
doe.
gov/info/FxananassaRoyalRoyce_v1_0;https://datadryad.
org/stash/dataset/doi:10.
25338/B8TP7GQuantity: 1 plate
Array: Genotyping
Format: 384 HT-array Plate
Number of Arrays: 384 arrays
Species: Strawberry
Unit Size: 1 plate