Waters, 186009171, XBridge Protein BEH SEC Column, 125Å, 2.5 µm, 4.6 mm X 150 mm, 1/pk

XBridge Protein BEH SEC Column, 125Å, 2.5 µm, 4.6 mm X 150 mm Column, containing 2.5 µm particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 1,000 – 80,000 Daltons. Each batch is specifically QC tested using Waters BEH125 SEC Protein Standard (p/n: 186006519) to help ensure consistent performance for challenging size-based protein separations. Note: This XBridge Protein BEH SEC, 125Å Column is also available with a vial of BEH125 Protein Standard at no additional charge (p/n: 176004332) Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis

Specifications
Chemistry: SEC
Separation Mode: Gel Filtration (aqueous)
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 5500 psi (379 Bar)
Endcapped: No
Bonding Technology: Diol (OH)
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 80000
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 125 Å
QC Tested: Protein
Format: Online Column
Packing Solvent: 20% methanol in water
Surface Area: 220
System: UHPLC, UPLC, HPLC
Particle Technology: BEH
Technique: LC
USP Classification: L20, L33, L59
Inner Diameter: 4.6 mm
Length: 150 mm
Carbon Load: 12 %
eCord: No
UNSPSC: 41115711
Application: Protein
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge Protein BEH SEC Column, 125Å, 2.5 µm, 4.6 mm X 150 mm, 1/pk The XBridge Protein SEC BEH Column provides a number of noteworthy advantages and is capable of handling even the most difficult chromatographic tasks. The column's exceptional mobile-phase pH stability will benefit you, contributing to a longer column lifetime. The XBridge Protein SEC BEH Column has exceptional particle efficiency, peak form, and capacity, ensuring test robustness. It also has high column reliability. The careful and deliberate design of the XBridge BEH HPLC Columns will result in an increase in productivity. The Columns are adaptable and can be used in your lab to develop cutting-edge LC-MS assays or quality-control procedures. The 2.5µm particle XBridge Protein BEH SEC, 125 Column uses the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as our popular line of HPLC-based SEC columns. They work best for separating proteins ranging in size from 1,000 to 80,000 Daltons. This XBridge Protein BEH SEC, 125 Column is also available with a free vial of BEH125 Protein Standard (PN 176004332). Utilizing the XBridge Protein BEH SEC Column will allow you to switch to UPLC Technology with ease. The XBridge Protein BEH SEC Columns are manufactured in state-of-the-art Waters specialized manufacturing facilities. These facilities follow strict industrial procedures to ensure that the highest quality standards are met and that equipment is built in accordance with ISO9001 and cGMP guidelines. You can view our collection of XBridge columns in our online catalog, along with the Waters' entire offerings, and shop for lab equipment on our website as per your lab needs. If you require assistance with any equipment or have any questions, please contact a member of our global support team members. You may also be interested in checking out the BEH200 SEC Protein Standard Mix ; The BEH200 SEC Protein Standard Mix is a five-component protein mixture (thyroglobulin, IgG, BSA, myoglobin, and uracil) used to calibrate ACQUITY UPLC BEH200 SEC, 1.7 µm Columns. It is recommended that this standard, like the QCRM product line, be used on a regular basis for benchmarking column and system performance.

FAQ
What is The Optimal pH Range For The XBridge Protein BEH SEC Column? A pH range of 2.5 to 8, a maximum pressure of 6500 psi (or 448 Bar), and a temperature of 60 C are ideal operating conditions for the XBridge Protein BEH SEC Column. Please keep in mind that using a higher pH may shorten the column's lifespan and functionality.