Waters, 186009254, nanoEase M/Z Peptide CSH C18 Column, 130Å, 1.7 µm, 300 µm X 50 mm, 1/pk

nanoEase M/Z Peptide CSH C18 Column, 130Å, 1.7 µm, 300 µm X 50 mm columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. The 300 µm ID columns are built utilizing a ultra low dispersion column design and offer the same simplicity of use as more traditional columns. Like all M/Z columns they withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters patented CSH Technology 130Å pore, C18, 1.7 µm particles. The first choice for peptide separations, CSH particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. They exhibit little dependence on strong ion pairing agents, making them ideal for LC or LC-MS applications. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) to before being QC accepted as a Peptide separation column. Suggested Uses: Bottom-up Proteomics, Nano-flow, Trap and Elute

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 11 pH
Maximum Pressure: 15000 psi (1034 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
QC Tested: Peptide
Format: Column
Surface Area: 185
System: Nano/Micro
Particle Technology: CSH
USP Classification: L1
Inner Diameter: 300 µm
Length: 50 mm
Carbon Load: 15 %
UNSPSC: 41115709
Application: Peptide
Brand: nanoEase M/Z
Product Type: Columns
Units per Package: 1 pk

Additional Information
nanoEase M/Z Peptide CSH C18 Column, 130Å, 1.7 µm, 300 µm X 50 mm, 1/pk The nanoEase M/Z Peptide CSH C18 Column is an exceptional, high-performance reversed-phase column that elevates the practice of liquid chromatography. With its distinct features and specifications, it is engineered to deliver precision and efficiency in a wide range of applications. The column's 130Å pore size guarantees good peak shape and resolution. This feature enables it to be the perfect lab equipment for the detailed analysis of peptides and proteins, helping to achieve accurate results every time. Integrated into this column are 1.7 µm particles, which have been carefully incorporated to offer high separation efficiency. This characteristic enables the nanoEase M/Z Peptide CSH C18 Column to separate even the most complex mixtures of peptides and proteins, ensuring a clear and precise analysis. Another impressive attribute of this column is its 300 µm inner diameter, that allows for high flow rates. This feature accelerates the analysis process, enhancing the overall efficiency of the liquid chromatography and saving valuable time in the lab. With a length of 50 mm, the nanoEase M/Z Peptide CSH C18 Column is engineered for long life. This longevity makes it not only a high-performing tool but also a cost-effective choice that withstands the test of time, making it a worthy investment. The nanoEase M/Z Peptide CSH C18 Column is end-capped to minimize MS bleed, a detail that aids in improving the sensitivity of your analysis. This feature provides researchers and lab technicians with reliable and consistent results, making the column a trusted tool for sensitive investigations. For alternative applications, try the nanoEase M/Z Peptide BEH C18 Column, 300Å, 1.7 µm, 75 µm X 200 mm, 1/pk . In summary, the nanoEase M/Z Peptide CSH C18 Column offers a host of benefits, including high separation efficiency, good peak shape, and resolution, high flow rates, long column life, and minimized MS bleed. With its versatility and reliability, this column is an ideal choice for a variety of liquid chromatography applications. Whether you're a seasoned researcher or a budding scientist, the nanoEase M/Z Peptide CSH C18 Column will be an indispensable tool in your quest for accurate and precise analysis.

FAQ
What is the retention time for peptides on the nanoEase M/Z Peptide CSH C18 Column? The retention time for peptides on the nanoEase M/Z Peptide CSH C18 Column will vary depending on the peptide sequence and the mobile phase used. However, in general, the retention time for peptides in this column is in the range of 1-10 minutes.