BD, 560760, BD Phosflow™ T Cell Kit Lyophilized Cells

Application: Intracellular staining (flow cytometry) (Routinely Tested)
RRID: AB_2869365
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store unopened vials of lyophilized cells at 2 - 8°C. Do not freeze. Vials should be opened and the lyophilized cells reconstituted just prior to use.
Recommended Assay Procedures: Recommended Assay Procedures 1.  Gently tap the vial on a flat surface before using. This ensures that the lyophilized cell pellet is on the bottom of the vial. 2.  Open a vial of BD Phosflow™ Treated or Untreated Human Control Cells. Reconstitute the cells in 120 uL of BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) at room temperature. Gently vortex the cells for 1 to 2 seconds and let stand for at least 5 minutes before use. 3.  Transfer 100 µL of control cells to a 12 × 75-mm polystyrene tube. Keep cells on ice until ready to stain. Add fluorescent antibodies specific for intracellular signaling proteins and cell surface antigens (ie, fluorescent antibodies listed in the figure legend that have been validated for these cells), vortex the tube, and incubate at room temperature for 1 hour, protected from light. 4.  Wash cells by adding 2 mL of Stain Buffer and immediately centrifuging them  (600g for 6 min), aspirate the supernatant, and resuspend the stained cells in 300 to 500 µL of Stain Buffer. Analyze the cells by flow cytometry immediately (optimal) or no longer than 4 hours after preparation. Keep the cells at 2 to 8°C and protected from light prior to data acquisition.
Product Notices: Product Notices This product contains human blood, serum, cells, or materials derived from them, which are potentially hazardous materials. Use universal precautions when handling. Handle as if product were capable of transmitting disease. Material used in this product has been tested using FDA approved methods and found negative for Human Immunodeficiency Virus (HIV-1/HIV-2), Hepatitis B Surface Antigen (HBSAG) and antibody to Hepatitis C Virus (HCV). However, no known test method can offer complete assurance that specimens of human origin will not transmit infectious disease. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen recommendations. Product Use Limitations: This product is for research use only. Research products are labeled as Research Use Only (RUO) and are not for use in diagnostic or therapeutic procedures. Customer agrees not to use the product for purposes which allow for any identification of the individual donors. Customer agrees to notify BDB Technical Services within five (5) business days of becoming aware of any identification or re-identification of an individual in connection with this product. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.