BD, 561338, BD Horizon™ V450 Mouse anti-Human IL-17F

Alternative Name: Interleukin-17F; ML-1; cytokine ML-1
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Human IL-17F
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_10642580
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed.
Recommended Assay Procedures: Recommended Assay Procedures This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells using BD Cytofix/Cytoperm™ reagents or BD™ Phosflow fixation and permeabilization buffers (Fix buffer I with Perm/Wash Buffer I, Perm Buffer II, or Perm Buffer III).
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Cy is a trademark of GE Healthcare. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.