BD, 556375, BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD178

Alternative Name: Fas Ligand, CD95 Ligand
Reactivity: Human (QC Testing)
Isotype: Mouse IgG2a
Immunogen: L51788Y Mouse T Lymphoma Cells Expressing Recombinant Human FasL
Application: Immunoprecipitation (Routinely Tested), Flow cytometry (Tested During Development), ELISA, Neutralization (Reported), Western blot (Not Recommended)
Target Molecular Weight: 40, 26 kDa
Concentration: 1.0 mg/ml
RRID: AB_396396
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures IP : Applications include immunoprecipitation (1-2 µg/one million cells). Functional Assay : NOK-2 has also been shown to neutralize the cytotoxic activity of FasL. Neutralization of FasL activity inhibits Fas-mediating  killing. The NA/LE format of NOK-2 (Cat. No. 556375) should be used for all functional assays. NOK-2 and a related human FasL clone, NOK-1 [Cat. No. 556371 (NA/LE)] may give different profiles in neutralization assays. It is thought that NOK-1 and NOK-2 likely recognize different FasL epitopes. Flow Cytometry : Another clone,  NOK-1, (556372, 556373)  are  recommended for flow cytometry. NOK-2 appears weaker for flow cytometry than NOK-1. Western Blot : Neither NOK-1 nor NOK-2 are suggested for western blot analysis. Another human FasL clone, G247-4 (Cat. No. 556387) is suggested for the western blot analysis.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.