BD, 560092, BD Phosflow™ PE Mouse anti-PDPK1 (pS241)

Alternative Name: PDK1 (pS241), PKB Kinase (pS241)
Reactivity: Human (QC Testing), Mouse, Rat (Predicted)
Isotype: Mouse IgG1, κ
Immunogen: Phosphorylated Human PDPK1 Peptide
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 20 µl
RRID: AB_1645523
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures This mAb was characterized by flow cytometry (Flow) and western blot analysis (WB) using these model systems: Method Species Cells Treatment Fixation Perm buffer Result Flow Human Jurkat Calyculin A + Okadaic Acid Lyse/Fix or Cytofix III Up-regulation Flow Human Jurkat Calyculin A + Okadaic Acid Lyse/Fix I or II Unsatisfactory Flow Human PBMC Calyculin A + Okadaic Acid Lyse/Fix III Up-regulation WB Human Jurkat Calyculin A + Okadaic Acid 63 kDa
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Source of all serum proteins is from USDA inspected abattoirs located in the United States. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.