BD, 563489, BD Phosflow™ PE Mouse Anti-mTOR (pS2448)

Alternative Name: Mammalian target of rapamycin; FRAP1; RAFT1; RAPT1
Reactivity: Human (QC Testing), Mouse, Rat (Predicted)
Isotype: Mouse IgG1, κ
Immunogen: Phosphorylated Peptide
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_2736872
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.