Thermo Fisher, 34580, SuperSignal™ West Pico PLUS Chemiluminescent Substrate Thermo Scientific™

SuperSignal™ West Pico PLUS Chemiluminescent Substrate Thermo Scientific™ Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:Sensitivity:pictogram to femtogram sensitivityStability:8-hr working solution stability; 1-year kit stability at room temperatureCompatibility:nitrocellulose and PVDF membranesSignal duration:6 to 24 hoursRecommended primary antibody concentration:1:1000–1:5000 dilution (0.
2–1.
0 μg/mL)Recommended secondary antibody concentration:1:20,000–100,000 dilution (10–50 ng/mL)Compare and learn more about our western blot chemiluminescent substratesView all HRP substrates availableSuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.
Conjugate: HRP
Kit Contents: SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide
Recommended Antibody Concentrations: 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.
2-1 μg/mL)
Sensitivity: Picogram to high femtogram-level
Shipping Condition: Room Temperature
Signal Duration: 6 to 24 hr.
Sufficient For: 60 Mini-Gel Blots, 5000 cm2of Membrane
Detection Method: Chemiluminescence
For Use With (Application): Western Blot
Form: Liquid
Membrane Compatibility: Nitrocellulose, PVDF
Product Line: SuperSignal™
Product Type: Substrate
Quantity: 500 mL
Substrate: Horseradish Peroxidase
Unit Size: Each