Merck, 11734334001, Universal Protease Substrate

The Universal Protease Substrate provides rapid and highly sensitive detection of trace protease activity, or determination of the effectiveness of protease inhibition. Protease activity releases resorufin-labeled peptides from the patented substrate, resorufin-labeled casein. Resorufin is N-(resorufin-4-carbonyl)-piperidine-4-carbonic acid).The concentration of these labeled peptides in the supernatant is directly related to the proteolytic activity present. •Conveniently detect nanogram quantities of proteolytic activity in less than one hour. •Perform highly sensitive protease detection in a homogeneous assay.