Abcam, ab100585, Human LYVE ELISA Kit

Size: 1 x 96Tests
Human LYVE ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human LYVE in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 8 pg/mL,
Range:8.19 - 2000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's Human LYVE ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human LYVE in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human LYVE coated on a 96-well plate. Standards and samples are pipetted into the wells and LYVE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LYVE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LYVE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein "LYVE1" also known as Lymphatic Vessel Endothelial Hyaluronan Receptor 1 has a molecular mass of about 60 kDa. This protein is located on the surface of lymphatic endothelial cells. Its expression occurs mainly within the lymphatic vessels serving as an important marker for identifying lymphatic endothelium. LYVE1 facilitates the transportation of hyaluronan an important glycosaminoglycan through lymphatic vessels playing a critical role in the maintenance of extracellular matrix and tissue fluid homeostasis.
Biological function summary
LYVE1 plays a role in lymphatic system regulation and immune cell trafficking. It forms part of a network that binds hyaluronan contributing to the regulation of cell migration and adhesion. This protein interacts with molecules like CD44 and other receptors which also participate in hyaluronan binding. LYVE1's function in maintaining the balance of hyaluronan within tissues is central affecting not only the lymphatic system but also immune responses.
Pathways
LYVE1 engages in both the hyaluronan metabolism and lymphangiogenesis pathways. Its interaction with CD44 plays a significant role in the transport and uptake of hyaluronan influencing cellular processes such as proliferation and motility. These interactions link LYVE1 to pathways controlling fluid balance and immune surveillance marking it as essential for maintaining healthy lymphatic and immune function.
LYVE1 shows a connection to lymphatic-related conditions such as lymphedema and cancer metastasis. Alterations or dysregulation of LYVE1 expression can affect lymphatic function potentially leading to the accumulation of interstitial fluid as seen in lymphedema. In cancer increased LYVE1 expression can relate to tumor metastasis where it functions alongside proteins like VEGFR-3 in promoting lymphatic vessel growth and facilitating cancer cell spread.