Abcam, ab100697, Mouse IL-10 ELISA Kit

Size: 1 x 96Tests
Mouse IL-10 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-10 in Mouse in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 45 pg/mL,
Range:78.13 - 5000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's Mouse IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of IL-10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C