Abcam, ab100718, Mouse Leptin ELISA Kit

Size: 1 x 96Tests
Mouse Leptin ELISA Kit is a sandwich ELISA designed to quantify Mouse Leptin with a sensitivity of 4 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 4.1 - 1000 pg/mL - Cited in over 30 publications
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 4 pg/mL,
Range:4.1 - 1000 pg/mL,
Assay Platform:Microplate

Product details:
Mouse Leptin ELISA Kit ab100718 is a sandwich ELISA to measure Mouse Leptin in serum, plasma, cell culture supernatant with a sensitivity of 4 pg/ml.
How the assay works
This assay employs an antibody specific for Mouse Leptin coated on a 96- well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Leptin ELISA Kit ab100718 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Leptin also known as the OB protein is a hormone with a molecular weight of approximately 16 kDa. Primarily it is produced by adipocytes in white adipose tissue and to a lesser extent in the placenta stomach and other tissues. This protein plays a significant role in energy homeostasis by conveying information to the central nervous system regarding fat storage. In scientific studies leptin is often measured using methods like the leptin ELISA kit or mouse leptin assay to monitor its levels and function.
Biological function summary
Leptin regulates food intake and energy expenditure by binding to its receptors in the hypothalamus. It is an important part of the feedback loop to manage body weight and appetite. Leptin is not part of a larger protein complex and acts directly on its target tissues. By signal transduction pathways it influences cellular processes such as metabolism and even impacts fertility and immune responses.
Pathways
Leptin is involved in the neuroendocrine signaling pathway and the MAPK/ERK pathway. In the neuroendocrine system leptin interacts with proteins like proopiomelanocortin (POMC) and neuropeptide Y (NPY) to manage appetite and energy use. Additionally it activates the JAK/STAT pathway when binding to its receptor which influences gene expression involved in metabolism and inflammation.
Leptin is linked to obesity and type 2 diabetes. Leptin deficiencies or resistance can lead to metabolic disorders as the hormone's signaling is disrupted affecting energy balance and storage. Its connection to adiponectin another hormone derived from fat tissue is critical in understanding these conditions; while leptin promotes inflammation adiponectin has anti-inflammatory effects. Exploring these proteins' roles and their balance offers insights into treatments for obesity-related disorders.