Abcam, ab100943, Recombinant Human RCL protein

Size: 100µg
Recombinant Human RCL protein is a Human Full Length protein, in the 1 to 174 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:O43598,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The reactive center loop (RCL) is an important structural element found in serpins including alpha-1 antitrypsin. It functions by acting as a bait for target proteases and then trapping them modifying their activity in proteolytic cascade reactions. The RCL's standard molecular mass is around 10 kDa but it is most often part of larger serpin proteins. RCLs are found in a variety of tissues prominently in the liver where they play a role in regulating enzymes in the blood.
Biological function summary
The reactive center loop operates by temporarily interacting with proteases to regulate their activities. It often finds itself as a component of a serpin-protease complex where it changes the enzymatic activity. This precise control allows proper regulation of proteolytic pathways that manage diverse physiological processes including inflammation and coagulation.
Pathways
Reactive center loops engage in the regulation of two main pathways: the coagulation cascade and the inflammatory response pathway. In these pathways RCLs work closely with proteins like thrombin and plasminogen to maintain a balance between enzyme activation and inhibition. Through these interactions RCLs help modulate the clot formation and dissolution as well as inflammatory response to injury.
Reactive center loops have ties to alpha-1 antitrypsin deficiency and emphysema. Aberrant RCL function can lead to inefficient enzyme inhibition contributing to these conditions. The protein alpha-1 antitrypsin which includes the RCL when mutated or deficient can result in unregulated neutrophil elastase activity causing tissue damage and related respiratory conditions.