Abcam, ab10140, Anti-SIRT4 antibody

Size: 100µg
Goat Polyclonal SIRT4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human SIRT4 aa 300 to C-terminus.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SIRT4 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9Y6E7

Product details:
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIRT4 also known as Sirtuin 4 is a mitochondrial protein with a mass of approximately 36 kDa. It belongs to the sirtuin family of NAD+-dependent deacetylases. Unlike many other sirtuins SIRT4 does not function as a deacetylase; instead it exhibits ADP-ribosyltransferase activity. This activity regulates a range of mitochondrial functions. SIRT4 is expressed in various tissues including the liver pancreas and muscles where it plays different roles based on the tissue type.
Biological function summary
SIRT4 regulates energy metabolism and cellular stress responses. It plays key roles in the regulation of mitochondrial functions during fasting and impacts gluconeogenesis and fatty acid oxidation. SIRT4 inhibits glutamate dehydrogenase (GDH) affecting amino acid metabolism and insulin secretion. By acting in this capacity SIRT4 controls the flow of nutrients and energy homeostasis exhibiting its critical role in metabolic regulation.
Pathways
SIRT4 integrates into a broader metabolic network including the insulin signaling and AMPK pathways. It interacts with proteins like GDH and AMPK influencing mitochondrial energy output and stress response. This relationship places SIRT4 in an important position for maintaining energy balance and responding to cellular metabolic demands.
SIRT4 links to metabolic conditions such as diabetes and metabolic syndrome. Dysregulation of SIRT4 activity can lead to impaired insulin secretion and glucose intolerance. Furthermore SIRT4's interaction with other sirtuins particularly SIRT1 suggests a functional interplay in protecting against metabolic stress and possibly influencing the development of these metabolic disorders.