Abcam, ab103059, Recombinant Human NUDT16 protein

Size: 100µg
Recombinant Human NUDT16 protein is a Human Full Length protein, in the 1 to 195 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q96DE0,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NUDT16 also known as diphosphatase is an enzyme that belongs to the nudix hydrolase family. It has a molecular mass of approximately 19 kDa. This enzyme cleaves diphosphate groups from nucleoside diphosphate derivatives. NUDT16 is highly expressed in several tissues including liver and testis. Its activity is important for maintaining cellular nucleotide pool balance by removing non-canonical nucleotides that can potentially be harmful if incorporated into DNA or RNA.
Biological function summary
NUDT16 functions to regulate nucleotide metabolism. It catalyzes the hydrolysis of nucleoside diphosphates like diadenosine tetraphosphate into monophosphates. NUDT16 often operates with other proteins in multi-enzyme complexes where it plays a role in modulating the concentrations of nucleoside diphosphates within cells. This activity coordinates the synthesis and degradation of nucleotides influencing cellular energy status and coenzyme levels.
Pathways
With regards to pathways NUDT16 integrates into nucleotide catabolism and salvage pathways. These pathways facilitate the conversion and recycling of nucleotides ensuring a steady supply for nucleic acid synthesis. NUDT16 interacts within these pathways alongside other key enzymes such as IMP dehydrogenase and GMP synthetase highlighting its role in controlling guanine nucleotide levels. This involvement provides a direct link to DNA replication and repair mechanisms which depend on the accurate control of nucleotide pools.
NUDT16 has connections to cancer and autoimmune diseases. Abnormal activity or expression of NUDT16 may contribute to the accumulation of atypical nucleotides potentially leading to mutations and genomic instability that characterize several cancer types. In autoimmune contexts altered NUDT16 function can impact immune cell activation and response. It exhibits an intriguing relationship with proteins like SAMHD1 a known regulator of nucleotide pools involved in immune responses and cancer.