Abcam, ab104635, Recombinant Human MTHFS protein

Size: 100µg / 500µg
Recombinant Human MTHFS protein is a Human Full Length protein, in the 1 to 203 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P49914,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 30% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MTHFS also known as methenyltetrahydrofolate synthetase is an enzyme that plays an important role in folate metabolism. It catalyzes the conversion of 5-formyltetrahydrofolate (5-formyl-THF) to 510-methenyltetrahydrofolate (510-methenyl-THF). MTHFS is a protein with a molecular mass of approximately 23 kDa. Expression occurs in various tissues with significant presence in the liver where folate-mediated one-carbon metabolism is important for various cellular processes.
Biological function summary
MTHFS impacts the cellular folate pool by regulating the balance of folate derivatives required for nucleotide biosynthesis and amino acid metabolism. It does not function as part of a larger protein complex but exerts its effects by modulating the availability of different folate forms. This balance is essential for the synthesis of purines and thymidylate key building blocks of DNA and RNA influencing cell proliferation.
Pathways
MTHFS plays a role in the folate cycle and the related one-carbon metabolism pathway. These pathways are critical for DNA synthesis and repair as well as methylation reactions. MTHFS interacts indirectly with enzymes such as dihydrofolate reductase (DHFR) and serine hydroxymethyltransferase (SHMT) which are also key players in these pathways. The precise regulation of these pathways reflects the importance of folate in maintaining cellular health and function.
Impaired MTHFS function can lead to disruptions in folate metabolism contributing to the development of conditions such as neural tube defects and certain types of anemia. Research suggests a link between altered MTHFS activity and increased risk of these disorders. Its relationship with other proteins involved in folate metabolism such as folate receptor 1 (FOLR1) highlights the broader impact of disrupted folate metabolism on disease mechanisms.