Abcam, ab104902, Anti-DMPO Nitrone Adduct antibody [N1664A]

Size: 100µg
Mouse Monoclonal DMPO Nitrone Adduct antibody. Suitable for WB, IP, ELISA, ICC/IF and reacts with Chemical samples. Cited in 4 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to DMPO Nitrone Adduct.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:N1664A,
Isotype:IgG1,
Carrier free:No,
Applications:WB, ICC/IF, ELISA, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Detects ~90kDa. Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.05% Sodium azideConstituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The DMPO nitrone adduct also known as 55-Dimethyl-1-Pyrroline N-Oxide adduct plays an important role in trapping free radicals during biological reactions. As a spin trap DMPO captures highly reactive radicals like hydroxyl and superoxide radicals converting them to more stable radical adducts that can be detected by Electron Spin Resonance (ESR) spectroscopy. The mass of the DMPO nitrone adduct varies depending on the specific radical bound but the core structure of DMPO nitrone has a molecular weight of 113.15 g/mol. DMPO adducts are expressed in conditioned systems and are especially useful in detecting radical species in cellular environments.
Biological function summary
DMPO nitrone adduct formation is critical for investigating oxidative stress within cells. It does not form part of a complex but acts as an independent trap for free radicals. By stabilizing reactive radicals DMPO adducts help to provide a clearer picture of the radical landscape within biological systems. This insight is essential for understanding the balance between reactive oxygen species production and antioxidant defense mechanisms in various cell types and tissues.
Pathways
DMPO nitrone adduct formation links closely to pathways involving oxidative stress and redox signaling. In these pathways DMPO assists in monitoring dynamic changes and interactions between radicals and antioxidant proteins like superoxide dismutase (SOD) and catalase. These pathways are important for maintaining cellular health and preventing oxidative damage.
The use of DMPO nitrone adduct is significant for research on oxidative stress-related conditions. It is connected to diseases like Alzheimer's and cardiovascular disorders where oxidative stress and free radical damage play a well-documented role. The formation of DMPO adducts provides a method for assessing the extent of radical involvement offering insights into disease mechanisms. The interaction between DMPO and proteins such as amyloid-beta in Alzheimer's highlights the importance of free radical monitoring in understanding disease progression.