Abcam, ab10538, Anti-Cdc27 antibody [AF3.1]

Size: 100µg
Mouse Monoclonal CDC27 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human CDC27 aa 800 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:AF3.1,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WB, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CDC27 aa 800 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P30260

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cdc27 also known as cell division cycle 27 or APC3 plays an important role in cell cycle regulation. It weighs approximately 92 kDa and is expressed in various tissues although it is found predominantly within the nucleus of proliferating cells. Cdc27 is a component of the anaphase-promoting complex/cyclosome (APC/C) which is vital for progression through mitosis and the G1 phase of the cell cycle by targeting specific proteins for degradation.
Biological function summary
Cdc27 serves as an essential part of the APC/C a large E3 ubiquitin ligase complex. By forming the scaffold for other subunits Cdc27 helps regulate the ubiquitination and subsequent degradation of mitotic cyclins and other proteins involved in cell cycle progression. The interaction of Cdc27 with other proteins like Cdc16 and Cdc23 stabilizes the APC/C complex ensuring its proper function.
Pathways
Cdc27 participates actively in the cell cycle especially during the G2/M transition and mitotic exit. It is involved in the ubiquitin-proteasome pathway where it intersects with proteins like securin and cyclin B1. These interactions allow Cdc27 to mediate the transition between cell cycle phases and support chromosome segregation.
Disruptions in Cdc27 expression or function are linked to cancer development particularly breast and colorectal cancers. Abnormal activity in the APC/C complex can lead to uncontrolled cell proliferation. Cdc27 works alongside Cdh1 another APC/C co-activator protein highlighting its significance in maintaining cell cycle fidelity.unger.