Abcam, ab10558, Anti-CD45 antibody

Size: 100µg
Anti-CD45 antibody (ab10558) is a rabbit polyclonal antibody detecting CD45 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P . Suitable for Human, Mouse, Rat . - Over 450 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-CD45 antibody (ab10558) is a rabbit polyclonal antibody and is validated for use in Flow Cyt (Intra), IHC-P, WB in human samples.
Anti-CD45 antibody (ab10558) has been cited over 450 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-CD45 antibody (ab10558) has high sensitivity and specificity.
Anti-CD45 antibody (ab10558) has 56 independent reviews from customers.
Anti-CD45 antibody (ab10558) specifically detects CD45 (UniProt ID: P08575; Molecular weight: 145kDa) and is sold in 100 µg selling sizes.
CD45, also known as the leukocyte common antigen, is a transmembrane protein tyrosine phosphatase crucial for immune cell signaling. It exists in multiple isoforms, such as CD45RA and CD45RO, which help distinguish between different immune cell types and their functional states. CD45 plays a significant role in regulating lymphocyte development, activation, and homeostasis. Alterations in CD45 expression or function are linked to various immunodeficiencies and autoimmune disorders, highlighting its importance in maintaining immune balance. Autoimmune diseases related to CD45 involve alterations in the expression or function of this protein, which can disrupt normal immune cell signaling and lead to immune system dysregulation.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD45 also known as Leukocyte Common Antigen (LCA) or protein tyrosine phosphatase receptor type C (PTPRC) plays an important role in regulating immune cell signaling. It is a transmembrane protein with a molecular mass around 180-240 kDa due to different isoforms produced by alternative splicing. This protein is expressed on all nucleated hematopoietic cells including T cells B cells and NK cells. CD45 differentiates leukocytes from other human cell types making it an important marker in immunological studies.
Biological function summary
CD45 functions as a regulator of tyrosine phosphorylation important for leukocyte signaling. It is not part of a complex but it acts independently to modulate receptor signaling thresholds. CD45 dephosphorylates Src family kinases which initiates T cell and B cell antigen receptor signaling. This regulation is necessary for lymphocyte maturation activation and homeostasis indicating CD45's significant role in the immune response.
Pathways
CD45 influences both the T-cell receptor (TCR) and B-cell receptor (BCR) signaling pathways. It impacts the Src family kinases including Lck and Fyn in T cells allowing efficient signal transduction following antigen recognition. The protein also aids in down-regulating signals from various immune checkpoints impacting activation pathways through related proteins like CD3 and CD19. By influencing these pathways CD45 ensures proper lymphocyte function and immune response modulation.
CD45 is closely linked to autoimmune diseases and leukemias. Its altered expression or mutation can result in improper immune system function contributing to autoimmunity as seen in rheumatoid arthritis. Furthermore aberrations in CD45 are associated with chronic lymphocytic leukemia where it impacts leukocyte differentiation and survival. Through its connections to Src family kinases in these conditions CD45 plays a critical role in disease development and progression making it a target for diagnostic and therapeutic strategies.