Abcam, ab106625, Anti-GATA3 antibody

Size: 100µg
Rabbit Polyclonal GATA3 antibody. Suitable for WB and reacts with Human samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human GATA3.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human GATA3. The exact immunogen used to generate this antibody is proprietary information.P23771,
Specificity:At least two isoforms of GATA3 are known to exist; ab106625 will recognize both isoforms.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GATA3 also known as GATA-binding factor 3 is a transcription factor with a molecular mass of about 50 kDa. It belongs to the GATA family of zinc finger transcription factors. GATA3 is mainly expressed in the immune system especially in T-cells as well as in the nervous system and mammary glands. It plays a role in the regulation of critical developmental processes and cell lineage commitment through its binding to the 'GATA' motif in the promoter regions of target genes.
Biological function summary
GATA3 functions as a regulator of gene expression involved in various differentiation processes. It is not a solitary molecule; it interacts with other proteins to form transcriptional complexes. In T-helper type 2 (Th2) cells GATA3 is necessary for Th2 cell differentiation and cytokine production. Its expression is vital for the development of the nervous system and mammary gland morphogenesis highlighting its role in diverse biological contexts.
Pathways
GATA3 plays a significant role in the Th2 differentiation pathway and is linked to the immune response. It acts in association with other transcription factors like STAT6 and T-bet. GATA3 promotes the transcription of Th2 cytokines such as IL-4 IL-5 and IL-13 while suppressing Th1 responses. This balance orchestrated by GATA3 is important for immune homeostasis and the body's ability to respond to infections and allergens.
The dysregulation of GATA3 is associated with atopic diseases and breast cancer. Aberrant expression of GATA3 is often observed in allergic conditions due to its role in Th2 cell differentiation and cytokine expression. In the context of breast cancer GATA3 levels can impact tumor progression and are correlated with estrogen receptor status. Additionally its relation with other breast cancer-related proteins like L50 underlines its importance in cancer biology and potential as a therapeutic target.