Abcam, ab108666, Testosterone ELISA Kit

Size: 1 x 96Tests
Testosterone ELISA kit is a competitive ELISA designed to quantify human testosterone in serum, plasma (citrate, heparin), and urine with a sensitivity of 0.07 ng/mL. - Wide dynamic range – quantifies 0.2–16 ng/mL - Designed to measure bound and free testosterone - High recovery rate – achieves 105.5% general recovery - Cited in over 55 publications
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum, Urine,
Reacts with:Human,
Assay type:Competitive,
Sensitivity:= 0.07 ng/mL,
Range:0.2 - 16 ng/mL,
Assay Platform:Microplate

Product details:
Testosterone ELISA Kit ab108666 is a competitive ELISA kit to measure human Testosterone (17β-Hydroxy-4-androstene-3-one) in serum, plasma (citrate, heparin), and urine with a sensitivity of 0.07 ng/mL.
How the assay works
Testosterone ELISA Kit ab108666 is a competitive ELISA. There are 2 types of competitive ELISAs:
antigen down
: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
antibody down
: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.
Assay specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
Testosterone ELISA Kit ab108666 protocol summary
Add standards or samples to the wells, incubate 2 hrs
Wash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr
Wash, then add 50 µl of SP Conjugate per well. Incubate 30 min
Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min
Add 50 µl of Stop Solution per well. Read at 450 nm immediately

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Testosterone also known as 17β-hydroxyandrost-4-en-3-one is a steroid hormone with a molecular mass of approximately 288.42 Da. It is primarily synthesized in the testes but also in smaller amounts in the ovaries and adrenal glands. Mechanically testosterone operates by binding to the androgen receptor a member of the nuclear receptor family which then modulates gene expression. Its synthesis is stimulated by luteinizing hormone (LH) which is released from the anterior pituitary gland.
Biological function summary
Testosterone plays an important role in the development of male secondary sexual characteristics such as increased muscle and bone mass and the growth of body hair. It's also part of the steroid hormone biosynthesis pathway influencing the production of estradiol through the aromatase enzyme. Testosterone contributes to the maintenance of reproductive tissues and is important for the modulation of mood and cognitive functions.
Pathways
Testosterone is integral to the HPG (hypothalamic-pituitary-gonadal) axis where it exerts feedback control by inhibiting the secretion of gonadotropin-releasing hormone (GnRH) and LH. Moreover testosterone is essential in the androgen receptor signaling pathway. This pathway involves other proteins like dihydrotestosterone (DHT) a potent androgen converted from testosterone via the 5α-reductase enzyme.
Low levels of testosterone commonly known as 'Low T' are linked to conditions like hypogonadism and certain metabolic syndromes. Testosterone levels can influence the risk of osteoporosis as sustained low levels reduce bone density. Additionally increased or decreased testosterone levels can pose risks to cardiovascular health with the target interacting with proteins such as sex hormone-binding globulin (SHBG) in these contexts.