Abcam, ab108685, Triiodothyronine (T3) ELISA Kit

Size: 1 x 96Tests
Triiodothyronine (T3) ELISA Kit is a competitive ELISA designed to quantify Triiodothyronine (T3) with a sensitivity of 0.05 ng/mL - Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Competitive,
Sensitivity:= 0.05 ng/mL,
Assay Platform:Microplate

Product details:
Abcam's Triiodothyronine (T3)
in vitro
competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of total Triiodothyronine in serum and plasma.
A 96-well plate has been precoated with anti-T3. Samples and the T3-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the addedT3-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Triiodothyronine (T3) also known as liothyronine is a thyroid hormone with a molecular mass of approximately 651.97 Da. It forms when enzymes called deiodinases especially in the liver and kidneys remove an iodine from thyroxine (T4). T3 is mainly expressed in cells of tissues with high metabolic activity including the heart liver and brain. In plasma T3 travels bound to proteins like thyroxine-binding globulin which helps maintain its stable concentration.
Biological function summary
T3 plays a central role in regulating metabolic processes and energy homeostasis. It acts on nearly every cell in the body influencing protein synthesis increasing basal metabolic rate and consequently affecting development growth and metabolism. T3 exerts its effects by binding to nuclear thyroid hormone receptors forming a hormone-receptor complex that regulates gene expression. This hormone engages in crosstalk with other signaling molecules linking it to broader metabolic networks.
Pathways
T3 integrates into the thyroid hormone signaling pathway influencing various metabolic and physiological functions. This pathway includes the conversion of T4 to T3 by iodothyronine deiodinases. T3 also interacts with the PI3K/Akt pathway an important signaling cascade involved in cellular survival and growth. Proteins like thyroid receptor alpha (TRα) and beta (TRβ) are vital in mediating the effects of T3 as they serve as binding sites for the hormone influencing the pathway's downstream function.
Alterations in T3 levels associate with conditions such as hyperthyroidism and hypothyroidism. Hyperthyroidism results from excess T3 leading to symptoms like increased heart rate and weight loss while hypothyroidism stems from T3 deficiency causing fatigue and weight gain. T3 plays a role in Graves’ disease an autoimmune disorder that can cause hyperthyroidism with thyroid-stimulating hormone receptor antibodies influencing the overproduction of the hormone. Dysregulation of T3 levels also impacts the cardiovascular system through its connection with proteins involved in heart function potentially contributing to heart disease.