Abcam, ab108698, Human Ferritin ELISA Kit

Size: 1 x 96Tests
Human Ferritin ELISA Kit is a sandwich ELISA designed to quantify Human Ferritin with a sensitivity of 0.53 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Cited in over 10 publications
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.53 ng/mL,
Assay Platform:Microplate

Product details:
Human Ferritin ELISA Kit ab108698 is a sandwich ELISA to measure Human Ferritin in serum, citrate plasma, heparin plasma with a sensitivity of 0.53 ng/ml.
How the assay works
A 96-well plate has been precoated with anti-Ferritin IgG antibodies. Samples, standards and the Ferritin-HRP conjugate are added to the wells, where Ferritin in the sample and standards binds to the precoated antibody and added Ferritin-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Ferritin in the sample and the intensity is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Ferritin ELISA Kit ab108698 protocol summary
1. Add samples, standards and controls to wells used.
2. Add prepared labeled HRPConjugate to each well. Incubate at room temperature.
3. After washing, add TMB substrate solution to each well. Incubate at room temperature.
4. Add Stop Solution to each well. Read immediately.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C