Abcam, ab108792, Mouse Albumin ELISA Kit

Size: 1 x 96Tests
Mouse Albumin ELISA Kit is a Sandwich ELISA used to quantify Mouse Albumin with a sensitivity of 0.45 ng/ml - Colorimetric ELISA kit - 450 nm readout. Works on any standard plate reader - Wide dynamic range – 0.781 - 200 ng/ml - Cited in over 95 citations
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:> 0.45 ng/mL,
Range:0.781 - 200 ng/mL,
Assay Platform:Microplate

Product details:
Mouse Albumin ELISA kit (ab108792) is a sandwich ELISA kit designed for the quantitative measurement of mouse Albumin levels in plasma, serum, urine, cell culture supernatant, cell lysate and tissue samples.
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Mouse Albumin ELISA Kit ab108792 is a competitive ELISA to measure mouse albumin in urine and cell culture supernatants with a sensitivity of > 0.45 ng/mL.
How the assay works
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.
Assay specificity
The assay has no reactivity with canine, bovine, equine, monkey, human, swine, rabbit albumin.
the reactivity with rat albumin is <10%
Mouse Albumin ELISA Kit ab108792 protocol summary
1. Add 25 µl of Standard or Sample and 25 µl of Biotinylated Protein per well. Incubate 1 hour.
2. Wash, then add 50 µl of SP Conjugate per well. Incubate 30 minutes.
3. Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 minutes.
4. Add 50 µl of Stop Solution per well. Read at 450 nm immediately.
How other researchers are using Mouse Albumin ELISA kit ab108792
Mouse Albumin ELISA kit ab108792 has been used to measure the Albumin Creatinin Ratio (ACR) in mice with polycystic kidney disease
and to assess hepatocyte derived organoids
References:
MS Tham etal., 2024.PMID:38191531,
J Liang et al., 2023, PMID:37907970
Get results in 90 minutes our SimpleStep ELISA® kit:
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Mouse Albumin ELISA kit
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is commonly used by researchers to measure liver or kidney function.
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ab282882
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These ELISA kits are also available for Rat:
- Rat Albumin ELISA kit
ab235642
- Rat ALT ELISA kit abab234579
- Rat AST ELISA kit
ab263883
Kidney function marker assays
- Creatinine Assay Kit
ab65340
– suitable for use as a serum creatinine assay and a urine creatinine assay
- Albumin (BCG) Assay Kit (Colorimetric)
ab235628
– suitable for use with serum
- Urea Assay Kit
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– suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.