Abcam, ab108816, Rat BNP 45 ELISA Kit

Size: 1 x 96Tests
Rat BNP 45 ELISA Kit is a sandwich ELISA designed to quantify Rat BNP 45 with a sensitivity of 7.9 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 15.625 - 1000 pg/mL - Cited in over 20 publications
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 7.9 pg/mL,
Range:15.625 - 1000 pg/mL,
Assay Platform:Microplate

Product details:
Rat BNP 45 ELISA Kit ab108816 is a sandwich ELISA to measure Rat BNP 45 in serum and plasma with a sensitivity of 7.9 pg/ml.
How the assay works
A BNP 45 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 45 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 45 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Rat BNP 45 ELISA Kit ab108816 protocol summary
1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.
There is significant cross-reactivity between BNP32 and BNP45.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols