Abcam, ab108837, Human Ferritin ELISA Kit

Size: 1 x 96Tests
Human Ferritin ELISA Kit is a sandwich ELISA designed to quantify Human Ferritin with a sensitivity of 0.42 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Wide dynamic range - quantifies 0.781 - 50 ng/mL - Cited in over 10 publications
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Plasma, Milk, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.33 ng/mL,
Range:0.781 - 50 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Human Ferritin ELISA Kit ab108837 is a sandwich ELISA to measure Human Ferritin in serum, plasma, cerebral spinal fluid (CSF), milk with a sensitivity of 0.42 ng/ml.
How the assay works
A Ferritin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ferritin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ferritin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Ferritin ELISA Kit ab108837 protocol summary
1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols