Abcam, ab108853, Human GC Globulin ELISA Kit (Vitamin D Binding Protein)

Size: 1 x 96Tests
Human GC Globulin ELISA Kit (Vitamin D Binding Protein) is a Competitive ELISA for the measurement of Human GC Globulin (Vitamin D Binding Protein) in Human in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Human,
Assay type:Competitive,
Sensitivity:= 67 ng/mL,
Range:0.098 - 100 µg/mL,
Assay time:3h,
Assay Platform:Microplate

Product details:
Abcam's Human GC Globulin (Vitamin D Binding Protein)
in vitro
competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of GC Globulin in plasma and serum.
A GC Globulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated GC Globulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of GC Globulin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols