Abcam, ab108893, Human Plasminogen ELISA Kit (PLG)

Size: 1 x 96Tests
Human Plasminogen ELISA Kit (PLG) is a sandwich ELISA designed to quantify Human Plasminogen (PLG) with a sensitivity of 0.29 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.625 - 40 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Serum, Cerebral Spinal Fluid, Milk, Saliva,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.29 ng/mL,
Range:0.625 - 40 ng/mL,
Assay time:3h,
Assay Platform:Microplate

Product details:
Human Plasminogen ELISA Kit (PLG) ab108893 is a sandwich ELISA to measure Human Plasminogen (PLG) in serum, plasma, cell culture supernatant, cerebral spinal fluid (CSF), urine, milk, cell lysate, tissue with a sensitivity of 0.17 ng/ml.
How the assay works
A Plasminogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Plasminogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Plasminogen captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Plasminogen ELISA Kit (PLG) ab108893 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin Peroxidase Conjugate. Incubate at room temperature.
4. Add Chromogen Substrate to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.
Get results in 90 minutes with our SimpleStep ELISA
Human Plasminogen ELISA Kit (
ab196262

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Plasminogen also known as PLG is an important protein with a molecular weight of approximately 92 kDa. Plasminogen is a zymogen which means it is an inactive precursor that requires activation to function. It is produced primarily in the liver and circulates in blood plasma. Its expression occurs mainly in the liver but you can detect it in various tissues including the kidney. Plasminogen binds to tissue surfaces and undergoes transformation into plasmin an active enzyme that plays significant roles in many biological processes.
Biological function summary
Plasminogen plays a central role in fibrinolysis which is the breakdown of fibrin clots. This process is essential in wound healing and maintaining normal blood flow. Plasminogen once activated to plasmin acts by degrading fibrin a major component of blood clots. The protein forms part of the fibrinolytic system along with other components like tissue plasminogen activator (tPA). Together they ensure that any clots forming within blood vessels are regulated and dissolved as necessary preventing blockages.
Pathways
Plasminogen is deeply involved in the coagulation and fibrinolytic systems. The conversion of plasminogen to plasmin is a critical step in these pathways requiring activators such as urokinase. Plasminogen has interactions with proteins like alpha-2-antiplasmin and thrombin which help modulate its activity. These interactions ensure a careful balance is maintained between clot formation and dissolution which is vital for normal bodily function.
Plasminogen deficiency is linked with conditions like ligneous conjunctivitis a rare disorder characterized by membranous lesions mainly in the eyes. Reduced plasminogen activity can lead to excessive clot formation contributing to thrombosis risk. Plasminogen's interaction with plasmin and its deficiency can also associate with angioedema a condition that causes sudden swelling in the deeper layers of skin and tissues. Understanding the pathways and the proteins involved in plasminogen's function presents therapeutic potential in managing these disorders.