Abcam, ab108895, Human PreAlbumin ELISA Kit (Transthyretin)

Size: 1 x 96Tests
Human PreAlbumin ELISA Kit (Transthyretin) is a sandwich ELISA designed to quantify Human PreAlbumin (Transthyretin) with a sensitivity of 49 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Wide dynamic range - quantifies 0.122 - 31.25 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Saliva, Urine, Plasma, Milk, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 49 pg/mL,
Range:0.122 - 31.25 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Human PreAlbumin ELISA Kit (Transthyretin) ab108895 is a sandwich ELISA to measure Human PreAlbumin (Transthyretin) in serum, plasma, cerebral spinal fluid (CSF), saliva, urine, milk with a sensitivity of 49 pg/ml.
How the assay works
A PreAlbumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PreAlbumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PreAlbumin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human PreAlbumin ELISA Kit (Transthyretin) ab108895 protocol summary
1. Add standard or sample to appropriate wells. Incubate at room temperature.
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature.
5. Add Chromogen Substrate to each well. Incubate at room temperature.
6. Add stop solution to each well. Read immediately

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Prealbumin also known as transthyretin is a protein with a molecular mass of approximately 55 kDa. The liver primarily synthesizes prealbumin and it is present in the bloodstream. Functionally prealbumin transports thyroxine and retinol-binding protein-retinol complex facilitating the distribution of these molecules to target tissues. As such it plays an integral role in maintaining retinol and thyroid hormone homeostasis.
Biological function summary
Prealbumin interacts with retinol-binding protein to form a complex essential for vitamin A transportation. This complex prevents renal filtration of retinol-binding protein thereby conserving vitamin A in circulation. The protein's role extends beyond transport as it influences neurophysiological processes due to its ability to bind thyroid hormones and affect gene expression related to metabolism. Prealbumin is primarily a part of the thyroxine-transferring complex reinforcing its critical position in hormonal and vitamin dynamics.
Pathways
Prealbumin's role in the thyroxine-binding globulin pathway highlights its importance in the endocrine system. Another pathway where prealbumin contributes significantly is the vitamin A metabolic pathway. In these pathways prealbumin maintains functional relationships with proteins like retinol-binding protein and thyroxine-binding globulin which jointly modulate thyroid hormone transport and vitamin A dynamics across cellular membranes.
Prealbumin directly associates with familial amyloid polyneuropathy and malnutrition. Familial amyloid polyneuropathy involves the deposition of prealbumin-derived amyloid fibrils in peripheral nerves impacting neuron function. In malnutrition decreased prealbumin levels serve as a biomarker reflecting poor nutritional status. Clinically prealbumin's relation to retinol-binding protein and thyroid hormone levels facilitates its use in diagnosing and monitoring these conditions.