Abcam, ab108907, Human Thrombin-Antithrombin Complex ELISA Kit (TAT)

Size: 1 x 96Tests
Human Thrombin-Antithrombin Complex ELISA Kit (TAT) is a sandwich ELISA designed to quantify Human Thrombin-Antithrombin Complex (TAT) with a sensitivity of 0.86 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 1.48 - 120 ng/mL - Cited in over 10 publications
Key facts
Detection method:Colorimetric,
Sample types:Milk, Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.86 ng/mL,
Range:1.48 - 120 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Human Thrombin-Antithrombin Complex ELISA Kit (TAT) ab108907 is a sandwich ELISA to measure Human Thrombin-Antithrombin Complex (TAT) in plasma, milk with a sensitivity of 0.86 ng/ml.
How the assay works
A Thrombin-Antithrombin Complex specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin-Antithrombin Complex specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin-Antithrombin Complex captured in plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Thrombin-Antithrombin Complex ELISA Kit (TAT) ab108907 protocol summary
1. Add standards or samples to appropriate wells and incubate for 2 hours
2. wash
3.Add Biotinylated Thrombin-Antithrombin Complex Antibody to each well and incubate for 1 hour
4.Wash
SP Conjugate to each well and incubate for 20 minutes
5. Wash
6. Add Chromogen Substrate to each well and incubate for 20 min
7. Add stop soution and read at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Thrombin-Antithrombin Complex also referred to as TAT complex plays a significant mechanical role in regulating blood coagulation. It forms from the interaction of thrombin an enzyme and antithrombin a serpin inhibitor. Thrombin shows an approximate molecular mass of 36 kDa while antithrombin weighs around 58 kDa. This complex is expressed in the liver and circulates in the bloodstream where it helps maintain hemostasis by neutralizing excess thrombin preventing unwanted clotting events.
Biological function summary
The complex participates in the regulation of the coagulation cascade. It functions as an essential inhibitor forming part of a larger set of interactions that ensure blood coagulation occurs correctly. As a result it plays a role in maintaining the balance between coagulation and anticoagulation reducing the risk of thrombotic conditions. The TAT complex can be detected using specific kits like TAT kits which measure its levels important for understanding coagulation status in conditions using both human and mouse models.
Pathways
The thrombin-antithrombin complex is integrally involved in the intrinsic and extrinsic pathways of the coagulation cascade. It serves as a feedback mechanism to control and limit thrombin activity. The balance it provides is important to the effective regulation of fibrin formation working alongside proteins such as fibrinogen and factor Xa. Antithrombin in particular requires heparin or heparin-like molecules to enhance its inhibitory effects on thrombin ensuring precise modulation of coagulation.
Thrombin-antithrombin complex levels correlate with various thrombotic and hemorrhagic disorders. Elevated levels often indicate ongoing activation of the coagulation system seen in conditions like deep vein thrombosis and disseminated intravascular coagulation (DIC). These elevated levels can result from increased thrombin generation and antithrombin consumption. The complex's interaction with proteins like factor V and fibrin is pertinent in the pathology of these disorders making it a valuable marker in diagnosis and monitoring therapeutic responses.