Abcam, ab108986, Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker

Size: 20µL / 100µL / 1mL
Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab108986) is a rabbit monoclonal antibody detecting PGP9.5 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 90 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4118,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), WB, IHC-P, IHC-Fr, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab108986) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, IP and WB.
Abcam's high quality manufacturing and validation processes ensure Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab108986) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab108986) has been confirmed by Western Blot testing in PGP9.5 knockout
ab263773
cells (
ab263773
Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab108986) has 11 independent reviews from customers.
Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab108986) specifically detects PGP9.5 (UniProt ID: P09936; Molecular weight: 25kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR4118 -
ab220823
Antibody clone EPR4118 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
647, Alexa Fluor
555, Alexa Fluor
488, Alexa Fluor
594, Alexa Fluor
568, APC (
ab196173
ab300549
ab302578
ab302664
ab305109
ab317591
PGP9.5, also known as UCH-L1/UCHL1, is a crucial protein in neuro research, particularly for its role as a neuronal and neuroendocrine marker. It is widely used to stain neuronal cell bodies and axons in both the central and peripheral nervous systems. Researchers use PGP9.5 to assess nerve fiber density and integrity, making it essential for studying neurodegenerative diseases, small fiber neuropathy and various cancers. Its reliable expression in neurons helps in evaluating neuronal health and pathology in different neurological conditions.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein Gene Product 9.5 (PGP9.5) also known as ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is an enzyme of approximately 27 kDa mass. PGP9.5 is highly expressed in neurons and neuroendocrine cells and is involved in the processing of ubiquitinated proteins. Ubiquitination and deubiquitination are critical for maintaining cellular protein homeostasis. PGP9.5 is present in the central and peripheral nervous systems. Researchers often utilize PGP9.5 as a neuronal marker due to its strong expression in nerve tissues. Antibody clone 13C4 is commonly used in PGP9.5 immunohistochemistry to visualize this protein in histopathology sections.
Biological function summary
PGP9.5 plays a significant role in the ubiquitin-proteasome pathway where it regulates protein degradation. It functions by cleaving ubiquitin from ubiquitin-protein conjugates which can recycle ubiquitin for reuse impacting protein turnover. This process is important for removing misfolded or damaged proteins maintaining cellular integrity. PGP9.5 has not been documented as a part of any large multimeric complex; its action is rather individual yet vital in cellular processes.
Pathways
PGP9.5 is an important participant in the ubiquitin-dependent proteolysis pathway. This pathway controls the degradation of proteins that regulate cell cycle differentiation and neuromodulation. PGP9.5 works in tandem with other proteins such as the proteasome complex to remove proteins tagged for destruction. Another important pathway linked with PGP9.5 is the neuronal development pathway in which its activity supports neuron growth and repair.
Defective PGP9.5 function associates mainly with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Mutations or dysregulation in PGP9.5 expression are implicated in the pathogenesis of these conditions affecting protein degradation and neuronal health. Other proteins like α-synuclein in Parkinson's disease interact with PGP9.5 pathways indicating their involvement in disease mechanisms. Researchers consider PGP9.5 a potential biomarker for neuronal pathology through methods like PGP9.5 IHC allowing for evaluation of nerve damage and neuroendocrine tumor diagnosis.