Abcam, ab109505, Anti-Tropomyosin 1 (alpha) antibody [EPR5158]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal TPM1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 5 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5158,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tropomyosin 1 (alpha) also known as alpha-tropomyosin is a protein widely expressed in skeletal and cardiac muscle tissues. This protein plays a central role in muscle contraction by binding to actin filaments. Its precise molecular weight is approximately 32.7 kDa. Alpha-tropomyosin stabilizes actin filaments and regulates the interaction of actin with myosin which is essential for muscular contraction.
Biological function summary
Alpha-tropomyosin ensures the regulation of actin-myosin interactions during muscle contraction. It forms part of the troponin-tropomyosin complex which includes other key proteins such as troponin I T and C. This complex undergoes conformational changes in response to calcium ion concentrations allowing muscle contraction to occur. Alterations in this complex can significantly affect muscle function highlighting the central role of alpha-tropomyosin.
Pathways
Alpha-tropomyosin operates within the actin cytoskeleton regulatory pathway and the calcium signaling pathway. It is closely related to the troponin complex proteins facilitating muscle contraction in these pathways by controlling actin and myosin interactions. Calcium ions bind to troponin C causing structural changes that shift tropomyosin on the actin filament therefore enabling the binding of myosin heads to actin.
Mutations or dysregulation of alpha-tropomyosin have been linked to conditions like hypertrophic cardiomyopathy and nemaline myopathy. These disorders often result from altered interactions within the troponin-tropomyosin complex and other proteins like myosin heavy chain. Defective regulation of these interactions impairs muscle contraction leading to the clinical manifestations seen in these diseases.