Abcam, ab109630, Anti-LIS1 antibody [EPR3335(2)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal LIS1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3335(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LIS1 also known as PAFAH1B1 is a protein with a molecular mass of approximately 45 kDa. It plays a significant role in cellular and developmental processes by stabilizing microtubules and facilitating intracellular transport. LIS1 expresses ubiquitously with notable presence in the brain and other neural tissues. It interacts with dynein motor complexes guiding the transport of cellular components along microtubules and is essential in mitosis and neuronal migration.
Biological function summary
LIS1 influences neuronal migration during brain development. It forms a complex with dynein and other proteins like NDEL1 which is important for distributing organelles and proteins in the neuron. This dynein-LIS1 complex ensures proper positioning of the nucleus during cell division and migration. It also regulates the alignment of chromosomes warranting correct cytoskeleton organization and cell polarity.
Pathways
LIS1 is deeply involved in neurodevelopmental pathways and the stabilization of the cytoskeleton. The protein is a central player in the Reelin signaling pathway which is essential for layering neurons during cortical development. LIS1 cooperates with other components such as the DISC1 and DCX to ensure proper functioning within these pathways. These interactions are vital for maintaining proper brain structure and neural circuitry.
LIS1 mutations are linked to lissencephaly a disorder characterized by abnormal neuronal migration resulting in a smooth brain structure. This condition stems from disruptions in the mechanisms regulating microtubule dynamics and nuclear positioning during neuronal development. The protein interacts with TUBA1A another protein implicated in lissencephaly affecting the stability and function of microtubules. Disorders such as Miller-Dieker syndrome also relate to abnormalities in LIS1 highlighting its critical role in neurodevelopment.