Abcam, ab110170, Mitochondria Isolation Kit for Cultured Cells

Size: 1Kit
Mitochondria isolation kit for cultured cells (ab110170) is designed for efficient isolation of mitochondria from cultured mammalian cells using differential centrifugation. Suitable for Western blot, immunocapture or activity assays. - Provides reagents for up to 20 isolations - Proven performance: cited in over 75 publications
Key facts
Assay time:1h,
Reacts with:Mammals

Product details:
ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Available with a dounce homogenizer as kit
ab110171
Mitochondrial isolation protocol summary:
- suspend cells in reagent A and incubate for 10 min
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant, repeat
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in reagent C and analyze or freeze

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C