Abcam, ab110226, Anti-HSV1 + HSV2 VP16 antibody [LP1]

Size: 100µg
Anti-HSV1 + HSV2 VP16 antibody [LP1] (ab110226) is a mouse monoclonal antibody detecting HSV1 + HSV2 VP16 in Western Blot, IP, ICC/IF . Suitable for Herpes simplex virus, Human herpesvirus 2 . - Over 20 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:LP1,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Herpes simplex virus, Human herpesvirus 2,
Applications:ICC/IF, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific for Herpes simplex virus tegument protein VP16. This antibody will cross react with HSV1 and HSV2.

Product details:
What is this antibody validated in?
Anti-HSV1 + HSV2 VP16 antibody [LP1] (ab110226) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Herpes simplex virus, Human herpesvirus 2 samples.
Trusted by the scientific community
Anti-HSV1 + HSV2 VP16 [LP1] (ab110226) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HSV1 + HSV2 VP16 also known as α-TIF (alpha trans-inducing factor) is an important transcriptional activator mechanically facilitating the initiation of the herpes simplex virus (HSV) transcription process. This protein typically measures around 491 amino acids in length and has a molecular mass of approximately 65 kilodaltons. VP16 is expressed within infected host cells where it interacts specifically with cellular factors to trigger transcription from the herpes simplex virus immediate-early genes a critical step in the viral replication cycle.
Biological function summary
HSV1 + HSV2 VP16 is essential in the lifecycle of HSV1 and HSV2 by acting as a regulator of gene expression. It functions as part of a larger multiprotein complex incorporating several host cellular components including HCF-1 and Oct-1 to modulate the transcription of viral genes. This regulation allows the virus to effectively control the expression of its genome directly impacting viral pathogenesis and replication.
Pathways
Proteins related to HSV1 + HSV2 VP16 are implicated in transcriptional pathways particularly those activating immediate-early genes in HSV. These pathways include the interactions with cellular proteins HCF-1 and Oct-1 alongside the cellular transcription apparatus. This system aligns with the broader regulatory framework of viral gene expression intersecting with essential pathways in the host to enable efficient viral replication and spread.
HSV1 + HSV2 VP16 contributes significantly to conditions related to herpes simplex virus infections including oral and genital herpes. This protein is a critical player in the reactivation of latent virus impacting the recurrence of disease in infected individuals. The interactions with proteins like HCF-1 and Oct-1 not only facilitate viral replication but also may influence host cellular pathways related to cell cycle and immune response potentially affecting the severity and progression of the disease.