Abcam, ab110294, Anti-ECH1 antibody [9D11AF3]

Size: 100µg
Mouse Monoclonal ECH1 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:9D11AF3,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
ECH1 is a member of the hydratase/isomerase superfamily involved in fatty acid metabolism. It is localized to peroxisomes and/or mitochondria in different mammals. In humans, levels of ECH1 are modulated by peroxisome proliferators. As the name implies, ECH1 catalyzes the isomerization of cis Δ3,5, double bonds in unsaturated acyl-CoAs to yield trans Δ2,4-dienoyl CoAs. These are substrates for Δ2,4-dienoyl CoA reductase which produces trans-3 enoyl CoA. This intermediate can then be further transformed by Δ3, Δ2 - enoyl CoA isomerase to finally yield the trans-2 double bond configured fatty acyl CoA, which can be then directed into the ß-oxidation pathway. Expression of ECH1 is highest in metabolically active tissues such as heart, skeletal muscle, central nervous system, liver and kidney. Recent studies show that it is down regulated in gastric carcinoma and CLL.
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Properties and Storage Information:
Form-Liquid, Purification technique-Precipitation Ammonium Sulphate, Purification notes-Near homogeneity as judged by SDS-PAGE. ab110294 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ECH1 also known as Delta(35)-Delta(24)-dienoyl-CoA isomerase is a mitochondrial protein with a mass of approximately 31 kDa. It is expressed in a wide variety of tissues including high levels in the liver heart and muscle. This protein plays an important role in the beta-oxidation of unsaturated fatty acids inside mitochondria by catalyzing the conversion of 35-dienoyl-CoA to 24-dienoyl-CoA. Because of this function ECH1 contributes to the efficient metabolism of fatty acids.
Biological function summary
The ECH1 protein facilitates the breakdown and utilization of unsaturated fatty acids thereby providing energy for cellular processes. It acts independently and is not a part of a large protein complex. Mitochondria rely on ECH1 to handle specific intermediates in fatty acid metabolism which impacts how cells manage their energy resources. This makes ECH1 essential for maintaining energy homeostasis particularly during periods of increased metabolic demand.
Pathways
ECH1 is integral to the mitochondrial fatty acid beta-oxidation pathway. This pathway is important for converting fatty acids into acetyl-CoA units which then enter the Krebs cycle for further energy production. ECH1 interacts with other proteins like ACAA2 which contributes to the final step of fatty acid degradation. Alongside this it aligns with the peroxisomal biogenesis pathway influencing the balance between metabolic processes within mitochondria and peroxisomes.
ECH1 activity impacts conditions such as metabolic syndrome and inherited disorders of mitochondrial fatty acid oxidation including Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency. Changes in the function or expression of ECH1 can lead to accumulation of fatty acid intermediates causing cellular dysfunction. In diseases where energy balance is disrupted like metabolic syndrome ECH1's interaction with proteins like HADHA which assists in the final steps of beta-oxidation becomes significant for understanding and potentially targeting treatments.