Abcam, ab110328, Anti-Frataxin antibody [18A5DB1]

Size: 100µg / 1mg
Anti-Frataxin antibody [18A5DB1] (ab110328) is a mouse monoclonal antibody detecting Frataxin in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human . - Over 30 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:18A5DB1,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, Flow Cyt, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Although this antibody does work in Western blot, we have found that
ab113691
typically gives a more robust signal. Therefore, if you plan on using this antibody in WB only, we recommend
ab113691
as an alternative.
Product Specifications
Anti-Frataxin antibody [18A5DB1] (ab110328) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-P, WB in human samples.
Anti-Frataxin antibody [18A5DB1] (ab110328) specifically detects Frataxin (UniProt ID: Q16595; Molecular weight: 19kDa) and is sold in 100 µg and 1 mg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Frataxin antibody [18A5DB1] (ab110328) has high sensitivity and specificity.
Anti-Frataxin antibody [18A5DB1] (ab110328) has been cited over 36 times in peer reviewed journals and is trusted by the scientific community.
Anti-Frataxin antibody [18A5DB1] (ab110328) has 5 independent reviews from customers.
Related Products
Antibody clone 18A5DB1 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488 (
ab156033
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Purification notes-ab110328 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Frataxin often known by the alternate name FXN is a mitochondrial protein with a mass of approximately 21000 Dalton. It is expressed mainly in tissues with high energy demands like the heart liver and pancreas. Frataxin plays an important role in iron-sulfur cluster assembly which is essential for various cellular processes. The protein is a part of mitochondria where it regulates iron homeostasis and prevents oxidative damage by minimizing iron-induced free radical generation.
Biological function summary
Several cellular processes depend on the correct function of this protein. Frataxin assists in forming iron-sulfur clusters acting within a multiprotein complex in the mitochondria. The complex includes proteins such as ISCU which are involved in the assembly and repair of iron-sulfur clusters. These clusters are necessary for supporting mitochondrial electron transport and other fundamental metabolic pathways that require iron-sulfur dependencies.
Pathways
Frataxin's involvement extensively affects the mitochondrial respiratory chain and the mitochondrial biogenesis process. It plays a role in the electron transport chain by stabilizing iron-sulfur-containing complexes. NAB is one associated protein that interacts closely within these pathways sharing a connection through iron-sulfur cluster transportation and assembly systems. Efficient function of these pathways ensures a proper energetic output of cells.
Frataxin mutations are directly linked to Friedreich's ataxia a neurodegenerative disease causing progressive damage to the nervous system. The deficiency or dysfunction in frataxin causes accumulation of iron in mitochondria leading to increased oxidative stress. Another related disorder includes heart disease which emerges due to the same oxidative stress pathway. Proteins such as Nfs1 are also involved sharing the responsibility with frataxin in scavenging excess iron protecting against related tissue damage.