Abcam, ab11039, Anti-PIP2 antibody [2C11]

Size: 100µg
Anti-PIP2 antibody [2C11] is a mouse monoclonal antibody is used to detect PIP2 in ICC/IF, IHC-P, ELISA and Neutralization. - Cited in over 20 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2C11,
Isotype:IgM,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:ELISA, IHC-P, FuncS (Neut/Block), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
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This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIP2 also known as phosphatidylinositol 45-bisphosphate is a minor phospholipid component found in the inner leaflet of the plasma membrane. It has a molecular mass of approximately 1000 Da. This lipid plays an important mechanistic role in cellular signaling by being a substrate for several important enzymes like phospholipase C (PLC) and phosphoinositide 3-kinase (PI3K). PIP2 is highly expressed in areas rich in cell signaling such as neurons and immune cells where it influences various cellular responses through its breakdown into second messengers.
Biological function summary
PIP2 participates in a wide range of cellular processes including the regulation of the actin cytoskeleton vesicle trafficking and ion channel function. As a part of the phosphoinositide signaling pathway PIP2 interacts with proteins to form complexes that alter cellular functions. For example it is involved in membrane ruffling and the internalization of membrane proteins. Through its conversion to diacylglycerol (DAG) and inositol trisphosphate (IP3) PIP2 activates protein kinase C (PKC) and calcium-dependent signaling influencing numerous physiological processes.
Pathways
PIP2 is embedded in several critical signal transduction pathways. Notably the phosphatidylinositol signaling system and the PI3K-AKT pathway link PIP2 with cellular proliferation survival and metabolism. Through enzymatic conversion PIP2 facilitates signal transduction cascades with proteins such as AKT and PTEN. These pathways play essential roles in ensuring proper cellular responses and maintaining homeostasis.
PIP2's altered metabolism or signaling can lead to various conditions such as cancer and neurodegenerative diseases. Cancer may arise due to deregulated PIP2 signaling and its effect on cell proliferation and survival with proteins like AKT being pivotal in this dysregulation. In neurodegenerative diseases like Alzheimer's PIP2 pathways may affect synaptic function and neuroinflammation linked with proteins including PLC. Understanding PIP2's involvement in these conditions aids in developing targeted therapies.