Abcam, ab110410, Complex II WB Antibody Cocktail

Size: 305µg
Complex II WB Antibody Cocktail (ab110410) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Reacts with:Mouse, Rat, Cow, Human,
Form:LiquidSee storage information,
Storage buffer:pH: 7.4Preservative: 0.02% Sodium azideConstituents: HEPES

Product details:
Contains a total of 305 μg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).
Cocktail Antibodies:
Mouse C-V-alpha [15H4C4] monoclonal
Amount: 50 μg
Working concentration: 1 μg/ml
Mouse C-II-30 (FeS) [21A11AE7] monoclonal
Amount: 250 μg
Working concentration: 5 μg/ml
Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal
Amount: 5 μg
Working concentration: 0.1 μg/ml
**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complex II also known as succinate dehydrogenase (SDH) or succinate-ubiquinone oxidoreductase plays an important mechanical role in the mitochondrial electron transport chain. It catalyzes the oxidation of succinate to fumarate while reducing ubiquinone to ubiquinol. The enzyme complex is approximately 140 kDa in mass and resides in the inner mitochondrial membrane. Complex II is expressed in most tissues particularly in high-energy demand tissues such as the heart and skeletal muscles.
Biological function summary
Complex II functions as part of the larger electron transport chain complex and plays a role in the Krebs cycle. It links two critical metabolic pathways converting succinate to fumarate while transferring electrons to the electron transport chain. This makes it integral for proper cellular respiration and energy production. The complex consists of multiple subunits and utilizes co-factors like FAD and iron-sulfur clusters for enzymatic activity. It is also a part of the supercomplexes that optimize the efficiency of oxidative phosphorylation.
Pathways
Complex II plays a significant role in both the citric acid cycle and oxidative phosphorylation. It acts as a connecting bridge between these two pathways facilitating the flow of electrons. Complex II works alongside other proteins such as complex I and complex III to maintain the electron transport chain's function and energy production. Succinate dehydrogenase transfers electrons within the chain directly affecting the generation of ATP by complex V (ATP synthase).
Dysfunction of complex II is associated with mitochondrial diseases and cancers. Mutations or deficiencies in its subunits can lead to conditions like Leigh syndrome and hereditary paraganglioma. These conditions frequently involve other mitochondrial proteins and complexes such as complex I which can exacerbate the electron transport chain dysfunction. In cancers alterations in succinate dehydrogenase activity can result in oncogenic metabolisms by falsely stabilizing hypoxia-inducible factors linking it further with the genetic and metabolic regulation.