Abcam, ab11304, Anti-alpha Tubulin antibody [B-5-1-2] - Microtubule Marker

Size: 50µL
Mouse Monoclonal Alpha-tubulin 4 antibody. Suitable for ICC and reacts with Human samples. Cited in 61 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human TUBA4A.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:B-5-1-2,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human TUBA4A.P68366,
Epitope:The antibody recognizes an epitope located at the C-terminal end of the alpha tubulin isoform.

Product details:
Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.
Biological function summary
Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.
Pathways
Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.
Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.