Abcam, ab113860, Recombinant Human GMDS protein

Size: 100µg
Recombinant Human GMDS protein is a Human Full Length protein, in the 1 to 372 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:O60547,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The GMDS protein also known as GDP-mannose 46-dehydratase plays an important role in catalyzing the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose one step in the biosynthesis of GDP-fucose. It has an approximate mass of 39 kDa and predominantly expresses in the liver kidneys and certain immune cells. The GMDS enzyme operates in the cytosol contributing to cellular metabolism and energy balance providing substrates necessary for further enzymatic conversions.
Biological function summary
GMDS contributes significantly to the fucosylation process a modification critical for the functioning of various glycoproteins and glycolipids. It participates in the synthesis of fucose which serves as a substrate for fucosyltransferases in glycan decorations. The enzymatic activity of GMDS occurs as part of a greater network within the cytosol although it does not directly involve multi-protein complexes. Its product GDP-fucose plays a role in cell-cell interaction and communication emphasizing GMDS’s importance in cell biology and molecular signaling.
Pathways
GMDS contributes to the fucose biosynthesis pathway and indirectly affects the Leloir pathway of galactose metabolism due to interconnected metabolic pools. Through its product GDP-fucose GMDS links to proteins such as fucosyltransferases which incorporate fucose into glycan structures. The availability of fucose also affects signaling pathways involving Notch receptor activation influencing cellular differentiation and growth making GMDS an essential player in glycan-related signal transduction.
GMDS alterations have connections with immunodeficiencies and various cancer types. Deficiencies in GMDS can lead to Congenital Disorder of Glycosylation Type Iic where improper glycosylation affects immune cell functions and fucosylation imbalance links to increased tumor progression and metastasis through altered cell surface glycan structures. In the context of these disorders GMDS influences proteins such as alpha-13-fucosyltransferases with fucose content affecting functional glycan patterns on cells demonstrating its importance in pathophysiology related to glycosylation disorders.