Abcam, ab113861, Recombinant Human Apolipoprotein A V/APOA5

Size: 100µg
Recombinant Human Apolipoprotein A V/APOA5 is a Human Full Length protein, in the 24 to 366 aa range, expressed in HEK 293 cells, with >70%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, ELISA, WB.
Key facts
Purity:>70% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:HEK 293 cells,
Tags:DDDDK tag C-Terminus,
Applications:WB, SDS-PAGE, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q6Q788,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in 200 µL of water,
Storage buffer:Constituents: 0.29% Sodium chloride, 0.24% Tris

Product details:
This product was previously labelled as Apolipoprotein A V

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein A V also known as APOA5 plays a critical role in lipid metabolism. This protein possesses a molecular weight of approximately 39 kDa and is mainly expressed in the liver. APOA5 modulates the plasma triglyceride levels by enhancing the uptake and clearance of triglyceride-rich lipoproteins. The protein is located at the surface of these lipoproteins facilitating their interaction with receptors and enzymes like lipoprotein lipase.
Biological function summary
APOA5 influences triglyceride metabolism and is part of the HDL particle complex. It interacts directly with lipoprotein lipase to promote the hydrolysis of triglycerides into free fatty acids. This hydrolytic action aids in reducing triglyceride levels in the plasma. APOA5 also indirectly influences very-low-density lipoprotein (VLDL) metabolism reinforcing its role in lipid regulation.
Pathways
APOA5 integrates into the lipoprotein metabolic pathway and the PPAR signaling pathway. In the lipoprotein metabolic pathway APOA5 works with proteins like APOC3 to influence triglyceride homeostasis. This protein is modulated by PPAR-alpha helping control lipid metabolism and energy homeostasis. Its role in these pathways highlights its impact on triglyceride-rich lipoprotein metabolism positioning it as an important player in maintaining lipid balance.
APOA5 strongly associates with hypertriglyceridemia and cardiovascular disease. Variations in the APOA5 gene can lead to abnormal plasma triglyceride levels contributing to the development of hypertriglyceridemia. Additionally APOA5's connection with APOE proteins through lipid metabolism pathways can influence susceptibility to cardiovascular disorders. Understanding the interactions and mutations within APOA5 provides insights into the mechanisms contributing to these disease states.