Abcam, ab113984, Anti-SETD1B antibody

Size: 100µg
Rabbit Polyclonal SETD1B antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SETD1B aa 350-400.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SETD1B aa 350-400. The exact immunogen used to generate this antibody is proprietary information.Q9UPS6

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab113984 is affinity purified using an epitope specific to SETD1B immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SETD1B also known as KMT2G is a histone methyltransferase protein with a molecular mass of approximately 200 kDa. It functions mechanically by specifically methylating lysine 4 on histone H3 (H3K4) a critical action for chromatin modification. This methylation regulates gene transcription and the enzyme predominantly exerts its effects in the nucleus. SETD1B expresses across various tissues which suggests a broad regulatory role in different cellular environments.
Biological function summary
The role of SETD1B extends to functioning as part of the SET1/MLL complex which is responsible for the mono- di- and tri-methylation of histone H3 at lysine 4 (H3K4). The complex plays a significant role in transcriptional regulation influencing gene activation and silencing across different cellular processes. SETD1B's activity modulates the expression of genes involved in the regulation of development differentiation and cellular proliferation impacting numerous biological outcomes.
Pathways
The involvement of SETD1B in gene expression regulation positions it notably in the Wnt signaling pathway. This pathway is important for controlling cell fate and proliferation. Furthermore SETD1B interacts with other proteins like β-catenin enhancing transcription of Wnt target genes. Additionally SETD1B takes part in the regulation of pathways involving chromatin remodeling associating closely with proteins such as ASH2L which affects histone methylation states.
Alterations in SETD1B are linked to intellectual disability and autism spectrum disorders. Disruptions in the protein's methylation function can lead to improper gene expression patterns emphasizing its association with neurodevelopmental conditions. SETD1B also connects with the CBP protein in certain cancer pathways where abnormal histone modification can drive oncogenesis. These interactions highlight SETD1B's significance in maintaining regular cellular function and its potential impact when mutant or dysregulated.