Abcam, ab114680, Recombinant Human hnRNP F protein

Size: 10µg
Recombinant Human hnRNP F protein is a Human Full Length protein, in the 1 to 415 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P52597,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.3% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Heterogeneous nuclear ribonucleoprotein F (hnRNP F) also known as hnRNP-F or HNRNPF plays an important role in RNA processing within the nucleus. It belongs to the family of hnRNPs which bind to nascent pre-mRNA molecules influencing their stability and transport. HnRNP F has a molecular weight of approximately 46 kDa. It is ubiquitously expressed found in various tissues but it exhibits higher levels of expression in the testis and brain. Its structured domains allow it to bind to RNA with high specificity impacting several RNA metabolic processes directly.
Biological function summary
HnRNP F interacts with RNA molecules to modulate alternative splicing mRNA stability and transport. It often forms part of larger ribonucleoprotein complexes that assemble on pre-mRNA. These complexes facilitate the accurate processing of pre-mRNA by guiding the necessary splicing machinery. HnRNP F commonly associates with other proteins such as hnRNP H enhancing its ability to regulate splice site selection and influencing mRNA diversity and function within the cell.
Pathways
HnRNP F is importantly involved in the regulation of gene expression through the mRNA splicing pathway. It particularly affects the spliceosome assembly a multi-protein complex essential for the removal of introns. hnRNP F's involvement in this pathway intersects with proteins such as SF2/ASF and U2AF65 which are key regulators in splicing. Furthermore hnRNP F influences the response to cellular stress pathways by stabilizing specific mRNAs under stress conditions often cooperating with proteins involved in stress granules.
HnRNP F has significant associations with neurodegenerative diseases and cancer. In neurodegenerative diseases aberrant splicing events influenced by hnRNP F are linked to conditions such as Alzheimer's disease where it interacts with splicing factors like SRSF1. In cancer altered expression or malfunction of hnRNP F contributes to splicing variants that promote oncogenesis notably in association with proteins like MYC which drives cancer cell proliferation. These connections make hnRNP F a potential target for therapeutic interventions in disease treatment.